Diabetic patients are frequently afflicted with impaired wound healing where linear progression of
molecular and cellular events compromised. Despite of meaningful progress in diabetic treatment,
management of diabetic chronic wounds is still challenging. Jamun (Syzygium cumini) honey may be a
promising candidate for diabetic wound healing and need to explore in detail.
So present study was designed to evaluate the efficacy of Jamun honey (JH) for diabetic wound healing
in in vitro wound (primary fibroblasts) model and in in vivo of diabetic mice (Streptozotocin induced)
model. The fibroblast cell model was studied for migratory behaviour and myofibrolasts infiltration
under honey interventions via scratch/migration assay, immuno-cytochemistry and western blot. We
applied FDA approved Manuka honey (MH) as positive control and JH as test honey to evaluate wound
re-epithelialization, sub-epithelial connective tissue modification and angiogenesis via histopathological and immuno-histochemical analysis. JH (0.1% v/v) dilution has notably improved wound
closure, migration with concomitant a-SMA expressions in vitro. Topical application of JH in diabetic mice
model showed significant (p
0.05) wound closure, reepithelialization, collagen deposition (I/III) and balanced the myofibroblasts formation. It also modulated vital angiogenic markers (viz HIF-1a, VEGF, VEGF ReII) significantly (p
0.05). All these observations depicted that JH promotes sequential stages of
wound healing in diabetic mice model. The results of the present study established Jamun honey as good
as Manuka honey considering wound closure, re-epithelialization, collagen deposition and proangiogenic potential.

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